Summary
The pantropical genus Psychotria L., probably the largest among angiosperms, occurs in different Brazilian vegetal formations. This work determined the chromosome numbers and karyotypic characteristics of ten species of Brazilian Psychotria. The chromosome numbers ranged from 2n = 22, in five species (P. hoffmannseggiana (Willd. ex Roem. & Schult.) Müll. Arg., P. lupulina Benth., P. marginata Sw., P. tenerior (Cham.) Müll. Arg., and P. trichophora Müll. Arg.), to 2n = 40, in P. mapourioides DC., and 2n = 44 in four other species (P. carthagenensis Jacq., P. gracilenta Müll. Arg., P. longipes Müll. Arg. and P. suterella Müll. Arg.). In addition to these differences, great variations in chromosome lengths and karyotypic formulae were also observed among these species. Chromosome length varied from 5.59 μm to 0.90 μm and no relationship was detected between chromosome numbers and lengths in any species. Chromosomes are principally metacentric, except for P. mapourioides that presents mainly submetacentric chromosomes. Karyotypic asymmetry rate (TF%) ranged from moderate (TF% = 38.83) to highly symmetric (TF% = 50.00). The difference in chromosome numbers of Psychotria carthagenensis (2n = 44) and P. mapourioides (2n = 40) is very important to taxonomists, because these have great difficulty in identifying and differentiating these two species with the sole use of morphological characteristics, since some individuals have intermediate characteristics between both species. Although this study does not allow for a consistent cytotaxonomic analysis, not even to delimit P. subgen. Psychotria and P. subgen. Heteropsychotria, this karyotype analysis of some Brazilian species, along with their morphology, may contribute to a better knowledge of the genus.
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References
Andersson, L. (2001). Margaritopsis (Rubiaceae, Psychotrieae) is a pantropical genus. Syst. Geogr. Pl. 71: 73 – 85.
____ (2002a). Re-establishment of Carapichea (Rubiaceae, Psychotrieae). Kew Bull. 57: 363 – 374.
____ (2002b). Relationships and generic circumscriptions in the Psychotria complex (Rubiaceae, Psychotrieae). Syst. Geogr. Pl. 72: 167 – 202.
____ & Rova, J. H. E. (1999). The rps16 intron and the phylogeny of the Rubioideae (Rubiaceae). Pl. Syst. Evol. 214: 161 – 186.
Bolkhovskikh, Z., Grif, V., Matvejeva, T. & Zakaryeva, O. (1969). Chromosome numbers of flowering plants. Academy of Sciences of the USSR, Leningrad.
Bremer, B. & Manen, J. F. (2000). Phylogeny and classification of the subfamily Rubioideae (Rubiaceae). Pl. Syst. Evol. 225: 43 – 72.
Corrêa, A. M. & Forni-Martins, E. R. (2004). Estudos cromossômicos em espécies de Rubiaceae (A. L. de Jussieu) de Cerrado. Caryologia 57(3): 250 – 258.
Davis, A. P., Bridson, D., Jarvis, C. & Govaerts, R. (2001). The typification and characterizations of the genus Psychotria L. (Rubiaceae). Bot. J. Linn. Soc. 135: 35 – 42.
Gill, L. S. & Obembe, A. O. (1991). Chromosome studies in some trees and shrubs from S Nigeria. Willdenowia 21: 233 – 238. http://mobot.mobot.org/W3T/Search/ipcn.html. (Accessed 2008 October).
Goldblatt, P. (1981). Index to plant chromosome numbers 1975 – 1978, vol. 5. Missouri Botanical Garden.
____ (1985). Index to plant chromosome numbers 1982 – 1983, vol. 13. Missouri Botanical Garden.
____ & Johnson, D. E. (1990). Index to plant chromosome numbers 1986 – 1987, vol. 30. Missouri Botanical Garden.
____ & Johnson, D. E. (1991). Index to plant chromosome numbers 1988 – 1989, vol. 40. Missouri Botanical Garden.
____ & ____ (1994). Index to plant chromosome numbers 1990 – 1991, vol. 51. Missouri Botanical Garden.
Guerra, M. (1983). O uso de Giemsa em citogenética vegetal — comparação entre a coloração simples e o bandamento. Ci. & Cult. 35: 190 – 193.
____ (1986). Reviewing the chromosome nomenclature of Levan et al. Revista Brasil. Genét. IX 4: 741 – 743.
Hamilton, C. W. (1989). A revision of Mesoamerican Psychotria subgenus Psychotria (Rubiaceae). Introductions and species 1 – 61. Ann. Missouri Bot. Gard. 76: 67 – 916.
Huziwara, Y. (1962). Karyotype analysis in some genera of compositae — VIII. Further studies on the chromosome of Asteride. Amer. J. Bot. 49: 116 – 119.
Kiehn, M. (1986). Karyosystematic studies on Rubiaceae: Chromosome counts from Sri Lanka. Pl. Syst. Evol. 154: 213 – 223.
____ (1996). Chromosome of Rubiaceae occurring in Malesia, the Philippines, New Guinea, and the Pacific. In: E. Robbrecht, C. Puff & E. Smets (eds), Second International Rubiaceae Conference Proceedings. Opera Bot. Belg. 7: 249 – 260.
Lopes, S. A., Moreno, P. R. H. & Henriques, A. T. (2000). Growth characteristics and chemical analysis of Psychotria carthagenensis cell suspension cultures. Enzyme and Microbial Technology 26: 259 – 264.
Nepokroeff, M., Bremer, B. & Systma, K. J. (1999). Reorganization of the genus Psychotria and the tribe Psychotieae (Rubiaceae) inferred from ITS and rbcL sequence data. Syst. Bot. 24: 5 – 27.
Petit, E. (1964). Les espèces africaines du genre Psychotria L. (Rubiaceae) — I. Bull. Jard. Bot. État 34: 1 – 229.
____ (1966). Les espèces africaines du genre Psychotria L. (Rubiaceae) — II. Bull. Jard. Bot. État 36: 65 – 190.
Pierozzi, N. I., Pinto-Maglio, C. A. F. & Cruz, N. D. da (1999). Characterisation of somatic chromosome of two diploid species of Coffea L. with acetic orcein and C-bands techniques. Caryologia 52: 1 – 8.
Pinto-Maglio, C. A. F., Pierozzi, N. I., Castro, S. C. P. & Soares-Scott, M. D. (1997). Números cromossômicos de Rubiaceae de Jundiaí — SP, Estação Experimental de Jundiaí, Instituto Agronômico de Campinas. International Organization of Plant Biosystematics 26/27.
Robbrecht, E. (1988). Tropical Woody Rubiaceae. Opera Bot. Belg. 1.
____ & Manen, J. F. (2006). The major evolutionary lineages of the coffee family (Rubiaceae, angiosperms). Combined analysis (nDNA and cDNA) to infer the position of Coptosapelta and Luculia, and supertree construction based on rbcL, rps16, trnL-trnF and atpB-rbcL data. A new classification in two sibfamilies, Cinchonoideae and Rubioideae. Syst. Geogr. Pl. 76: 85 – 146.
Sohmer, S. H. & Davis, A. P. (2007). The genus Psychotria (Rubiaceae) in the Philippine Archipelago. Sida, Bot. Misc. 27.
Steyermark, J. A. (1972). Rubiaceae. In: B. Maguire et al. The botany of the Guayana highland – part IX. Mem. N. Y. Bot. Gard. 23: 227 – 832.
Taylor, C. M. (1996). Overview of the Psychotrieae (Rubiaceae) in the Netropics. Opera Bot. Belg. 7: 261 – 270.
____ & Steyermark, J. A. (2004). Flora of the Venezuelan Guayana Volume 8 – Poaceae-Rubiaceae. Missouri Botanical Garden Press, St. Louis.
Acknowledgements
Instituto Florestal and Instituto de Botânica, São Paulo, Brazil are thanked for their permission to collect plants in protected cerrado areas. CNPq and FAPESP (03/02812 process) are acknowledeged for financial support.
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Corrêa, A.M., Jung-Mendaçolli, S.L. & Forni-Martins, E.R. Karyotype characterisation of Brazilian species of the genus Psychotria L. — subfamily Rubioideae (Rubiaceae). Kew Bull 65, 45–52 (2010). https://doi.org/10.1007/s12225-010-9176-5
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DOI: https://doi.org/10.1007/s12225-010-9176-5