Study on the Antinociceptive Activity and Mechanism of Action of Isolated Saponins from Siolmatra brasiliensis (Cogn.) Baill
Abstract
:1. Introduction
2. Results
2.1. Assessment of Side Effects and Toxicity
2.2. Antinociceptive Effect of HE, EtOAc, SI, D, B4, and A1 in the Hot Plate Test
2.3. Investigation of the Mechanism of Action of EtOAc, SI, D, B4, and A1 in the Hot Plate Model
2.4. Antinociceptive Effect of HE, EtOAc, SI, D, B4, and A1 in the Capsaicin- or Glutamate-Induced Nociception
3. Discussion
4. Methods
4.1. Plant Material
4.2. Extraction and Isolation
4.3. Animals
4.4. Extracts, Isolated Substances, and Control Drugs’ Administration
4.5. Drugs and Reagents
4.6. Acute Toxicity
4.7. Hot Plate Test
4.8. Analysis of the Mechanisms of Action of Hydroethanol Extract (HE), Ethyl Acetate Fraction (EtOAc), and its Isolated Saponins
4.9. Capsaicin- and Glutamate-Induced Nociception
4.10. Statistical Analysis
5. Conclusion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds hydroethanol extract (HE), ethyl acetate fraction (EtOAc), siolmatroside I (SI), cayaponoside D (D), cayaponoside B4 (B4) and cayaponoside A1 (A1) are available from the authors. |
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Giorno, T.B.S.; Santos, C.H.C.d.; Carvalho, M.G.d.; Silva, V.C.d.; Sousa, P.T.d., Jr.; Fernandes, P.D.; Boylan, F. Study on the Antinociceptive Activity and Mechanism of Action of Isolated Saponins from Siolmatra brasiliensis (Cogn.) Baill. Molecules 2019, 24, 4584. https://doi.org/10.3390/molecules24244584
Giorno TBS, Santos CHCd, Carvalho MGd, Silva VCd, Sousa PTd Jr., Fernandes PD, Boylan F. Study on the Antinociceptive Activity and Mechanism of Action of Isolated Saponins from Siolmatra brasiliensis (Cogn.) Baill. Molecules. 2019; 24(24):4584. https://doi.org/10.3390/molecules24244584
Chicago/Turabian StyleGiorno, Thais Biondino Sardella, Carlos Henrique Corrêa dos Santos, Mario Geraldo de Carvalho, Virgínia Cláudia da Silva, Paulo Teixeira de Sousa, Jr., Patricia Dias Fernandes, and Fabio Boylan. 2019. "Study on the Antinociceptive Activity and Mechanism of Action of Isolated Saponins from Siolmatra brasiliensis (Cogn.) Baill" Molecules 24, no. 24: 4584. https://doi.org/10.3390/molecules24244584